Fig. 3: AA rescues the action of AR signalling in C4-2 cells.

a Heat map from transcriptome analyses shows the hierarchical clustering of all identified differentially expressed genes (DEGs, adjusted p value < 0.05) upon AA alone, R1881 alone, AA + R1881 co-treatment, and the solvent control DMSO in all of the replicates. b Heat map of expression of known AR target genes plotted in all of the replicates and groups. c Normalised RNA counts of positively regulated AR-target genes in C4-2 cells upon AR ligand treatments. n = 3, for calculating the adjusted p value and normalised counts (DESeq2R package); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n.s., not significant. d Activity prediction of top 50 upstream regulators in R1881 + AA co-treatment compared to R1881 alone. Colour bar on the right side shows the −log 10 of enrichment p value (the redder, the more significant). The x-axis shows the activity prediction (Z-score) for each upstream regulator (using RNA-seq data, calculated by IPA). Positive Z-score shows activation and negative Z-score shows inhibition. Dashed line indicates the significance threshold, Z-score < −2 and Z-score> 2 are significant.