Fig. 5: AA inhibits angiogenesis in CRPC xenografted tumours. | Oncogene

Fig. 5: AA inhibits angiogenesis in CRPC xenografted tumours.

From: The natural compound atraric acid suppresses androgen-regulated neo-angiogenesis of castration-resistant prostate cancer through angiopoietin 2

Fig. 5

a SA β-Gal staining of native tumour slices of AA and mock-treated mice. b Immunofluorescence staining of C4-2 tumour tissues for proliferation marker Ki67, endothelial marker CD31 and Hoechst 33258. Tumour tissues were isolated from mice that were sacrificed on day 19. Representative images are shown. BF, bright-field; scale bars indicate 100 μm. c Ki67 staining of tumour tissues was quantified by ImageJ software. Obtained values were plotted representing the relative level of Ki67 expression per cell nucleus analysed, noted as of relative units (r.U.). 20 images were analysed per tissue and treatment. d Vessel numbers were measured by quantification of CD31 staining.

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