Fig. 5: Impact of Vav2Onc catalysis-dependent signaling in tumor stem cells.
From: The Rho guanosine nucleotide exchange factors Vav2 and Vav3 modulate epidermal stem cell function
A Penetrance of tumors in DMBA-treated mice of indicated genotypes. P < 0.001 (Chi–squared test, n = 13 and 15 WT and Vav2Onc/Onc mice, respectively). B Tumor type distribution of tumors at the endpoint of the experiment shown in A. P < 0.001 (Chi–squared test, n = 80 and 97 WT and Vav2Onc/Onc tumors, respectively). C Flow cytometry gating strategy used for the isolation of tumor stem cells. D Heatmap showing the transcripts that are differentially expressed between Vav2Onc/Onc and WT TSCs. The number of probe sets for the upregulated and downregulated fractions is indicated. E Main upregulated (red) and downregulated (blue) functional categories encoded by the transcriptome of Vav2Onc/Onc TSCs. For all of them, P < 0.01 (Fisher’s exact test). F GSEA of indicated gene sets in the Vav2Onc/Onc TSC transcriptome. The normalized enrichment scores (NES) and false discovery rate q–values (FDR q–val) are indicated within each graph. Positive and negative enrichments are indicated with upward and downward pointing arrows, respectively. G Survival plot of hnSCC patients (n = 97) according to the expression of the Vav2Onc-driven TSC transcriptional signature. The Mantel–Cox test P value is indicated.
