Fig. 1: ANKFN1 is frequently overexpressed and associated with a poor prognosis in HCC patients.
From: ANKFN1 plays both protumorigenic and metastatic roles in hepatocellular carcinoma
A ANKFN1 was found to be overexpressed in HCC tissues versus normal tissues according to analysis of TCGA HCC data using the R language package “limma”. B qRT–PCR analysis of ANKFN1 mRNA expression levels in HCC tissue compared with paracarcinoma tissue. C qRT–PCR analysis of ANKFN1 mRNA expression levels in HCC cells (SMMC-7721, HLE, Hep G2, HuH7, BEL-7404) compared with normal liver cells (LO2). D Representative images of ANKFN1 IHC staining in HCC tissues of high and low level. Original magnification: ×100. E HCC tissues stratified by the IHC staining index. F Kaplan–Meier analysis of the OS of 126 HCC patients stratified by ANKFN1 expression. ANKFN1 ankyrin repeat and fibronectin type III domain containing 1, HCC hepatocellular carcinoma, TCGA The Cancer Genome Atlas, qRT‐PCR quantitative real‐time polymerase chain reaction, IHC immunohistochemistry. *P < 0.05; **P < 0.01; ***P < 0.001.
