Fig. 2: Knockdown of ANKFN1 suppressed HCC cell growth through induction of G1-S cell cycle arrest and cell apoptosis.
From: ANKFN1 plays both protumorigenic and metastatic roles in hepatocellular carcinoma
A qRT‐PCR assay analyses of ANKFN1 expression levels in SMMC-7721 and HLE cells infected with sh-ANKFN1-RNA. B Immunofluorescence assay analyses of ANKFN1 expression levels in SMMC-7721 and HLE cells infected with sh-ANKFN1-RNA. Scale bars, 50 μm, 20 μm. C The effect of ANKFN1 knockdown on SMMC-7721 and HLE cell proliferation was assessed by the CCK-8 assay. D The effect of ANKFN1 knockdown on SMMC-7721 and HLE cell proliferation was assessed by the BrdU assay. E and F Flow cytometry analysis of cell apoptosis and cycle distribution in SMMC-7721 and HLE cells with or without ANKFN1 knockdown. ANKFN1 ankyrin repeat and fibronectin type III domain containing 1, HCC hepatocellular carcinoma, qRT‐PCR quantitative real‐time polymerase chain reaction, IHC immunohistochemistry. *P < 0.05; **P < 0.01; ***P < 0.001.
