Fig. 6: Overexpression of ANKFN1 promotes HCC migration and metastasis in vivo.
From: ANKFN1 plays both protumorigenic and metastatic roles in hepatocellular carcinoma
A Liver tissues from animals with tumor xenografts inoculated with SMMC-7721 cell lines stably expressing ANKFN1. B HE staining of orthotopic liver transplantation tumors. C IHC analysis of HE and Ki-67 expression levels in xenograft tumor tissues in the scramble and ANKFN1-overexpression groups. Scale bars, 200 μm. D Statistical graph of the number and area of liver metastases in nude mice. E TUNEL assay for cell apoptosis in tumor tissues developed from SMMC-7721 cells with or without ANKFN1 overexpression. Scale bars, 20 μm. F Western blot analysis of the expression of the MAPK signaling pathway proteins p-ERK1/2 and p-JNK1/2 and c-Myc, Cdk6, Cdk4, PCNA, and RhoA in SMMC-7721 and HLE cells with or without ANKFN1 overexpression. ANKFN1, ankyrin repeat and fibronectin type III domain containing 1, HCC hepatocellular carcinoma; *P < 0.05; **P < 0.01; ***P < 0.001.
