Fig. 7: ANKFN1 downregulation is mainly mediated by the downregulation of p-ERK in HCC cells.
From: ANKFN1 plays both protumorigenic and metastatic roles in hepatocellular carcinoma
A and B qRT–PCR analyses of the expression of ANKFN1 in LV-ANKFN1-SMMC-7721 cells after induction with the ERK inhibitor FR180204 at 48 h and 72 h. C The effect of FR180204 on SMMC-7721 cells infected with LV-ANKFN1 cell proliferation was assessed by the CCK-8 assay at 24 h, 48 h, and 72 h. D The effect of FR180204 on SMMC-7721 cells infected with LV-ANKFN1 cell proliferation was assessed by the BrdU assay at 72 h. E and F Flow cytometry analysis of the cell cycle distribution in SMMC-7721 cells infected with LV-ANKFN1 and induced with the ERK inhibitor FR180204 at 24 h, 48 h, and 72 h. G Western blot analysis of p-ERK, c-Myc, cyclin D1, Cdk4, Cdk6, and PCNA levels in SMMC-7721 cells infected with LV-ANKFN1 and induced with the ERK inhibitor FR180204 at 24 h, 48 h, and 72 h. *P < 0.05; **P < 0.01; ***P < 0.001.
