Fig. 8: A push and pull pathway model to explain high glutaminolysis flux in TNBC.
From: Glutamine addiction promotes glucose oxidation in triple-negative breast cancer
Single-pass glutaminolysis (orange) in TNBC cells is caused by (1) EAA aminotransferases withdrawing αKG and (2) alanine aminotransferase pushing glutamate into the TCA cycle. (3) αKG is drawn through the TCA cycle by citrate synthase assimilating glucose-derived acetyl-CoA (purple). The single-pass glutaminolysis flux was as large as the net deamidated flux of glutamine (green). (4) MCF-7 cells possessed a higher capacity for pyruvate anaplerosis that supplements αKG (blue), unlike TNBC cells that rely on glutamine to produce αKG.
