Fig. 5: miR-3591-3p suppresses tumor growth in vitro and in vivo by blocking cell cycle progression. | Oncogene

Fig. 5: miR-3591-3p suppresses tumor growth in vitro and in vivo by blocking cell cycle progression.

From: Tumor-derived exosomes deliver the tumor suppressor miR-3591-3p to induce M2 macrophage polarization and promote glioma progression

Fig. 5

A Flow cytometry analysis of cell cycle phase is shown of U118MG cells transfected with miR-NC or miR-3591-3p mimics. B Western blots were used to detect the levels of G2/M-related proteins. C Representative flow cytometry plots of cell apoptosis and quantitative analysis are shown. D Western blot analysis of apoptosis-associated proteins. E In vivo bioluminescent imaging assay of tumor burden in xenograft nude mice bearing U118MG pretreated with LV-miR-3591-3p or LV-NC. Representative images and quantifications are shown (n = 5 of each group). F Kaplan–Meier survival curves for overall survival in different groups (n = 10 of each group). G Representative HE staining of tumor tissues are shown from the two groups (scale bar, 2.5 mm). H Representative pictures of immunohistochemical staining of Ki67 and Bax are shown (scale bar, 100 μm). Data are shown as the mean ± SD of three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001).

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