Fig. 7: miR-3591-3p suppresses tumor via MAPK signaling pathway. | Oncogene

Fig. 7: miR-3591-3p suppresses tumor via MAPK signaling pathway.

From: Tumor-derived exosomes deliver the tumor suppressor miR-3591-3p to induce M2 macrophage polarization and promote glioma progression

Fig. 7

A Western blot results for the expression of proteins in the MAPK signaling pathway in U118MG cells transfected with miR-NC, miR-3591-3p mimics, inhibitor-NC, and miR-3591-3p inhibitor. B Western blot results for the expression of proteins in the MAPK signaling pathway in U118MG cells transfected with si-NC or si-MAPK1. C MAPK1 overexpressing plasmids were transfected following miR-3591-3p mimics transfection, and the MAPK signaling pathway-related proteins were detected by western blot in U118MG cells. The intensity of protein bands was quantified by densitometry. D Schematic diagram depicting the key assumptions and conclusions. Briefly, miR-3591-3p was excreted by glioma cells via exosomes and then taken up by macrophages. In macrophages, miR-3591-3p promotes macrophage to M2 phenotype by inhibiting the activity of CBLB and then activating JAK2/PI3K/AKT/mTOR and STAT3 pathways. On the other hand, since miR-3591-3p plays a tumor suppressor role in glioma cells, when it is excreted, the inhibitory effect on MAPK1 is relieved and thus promoting glioma progression by activating the MAPK pathway. Data are shown as the mean ± SD of three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001).

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