Fig. 7: CircVPRBP represses nodal metastasis of CCa in a RACK1-dependant manner.

A The schematic illustration of Tet-on induced RACK1 expression system (upper panel). Western blots analysis showed the RACK1 expression levels under indicated treatments (lower panel). B The workflow of the xenograft popliteal node metastasis model (upper panel). Representative images of popliteal LNs in different treatment groups (lower panel, n = 16). C The LNs volume were shown as the histogram analysis in different groups. D Representative images of immunostaining of pan-cytokeratin of popliteal LNs. Original magnifification, ×100. E Quantification of the rate of LN metastasis in the indicated groups. F, G LYVE-1 positive vessels in the footpad tumors of indicated groups as indicated by immunohistochemistry (F) and histogram analysis (G). H, I Tube formation by HLECs treated with conditioned medium collected from SiHa and MS751 cells under different treatments. Each experiment was performed at least three times independently. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.