Fig. 2: USP7 stabilizes the expression of SAMHD1. | Oncogene

Fig. 2: USP7 stabilizes the expression of SAMHD1.

From: De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity

Fig. 2

A Western blot analysis demonstrating gradually increased SAMHD1 protein levels with increased amounts of Flag-USP7 plasmids transfected. B The effect of three target sequences of siRNA-USP7 on the expression of SAMHD1 48 h after transfection. C Western blot analysis of SAMHD1 with or without P5091 treatment for 24 h at different concentrations. D, E Levels of SAMHD1 expression after different durations of CHX (20 μM) administration with or without MG132 (20 μM) treatment. Data are shown as mean ± SEM (**P < 0.01). F, G SAMHD1 expression after different durations of CHX (20 μM) administration in the cells 48 h after transfected with HA vector, HA-USP7 WT, or HA-USP7 C223S mutant plasmids. Data are shown as mean ± SEM (***P < 0.001). H, I SAMHD1 expression after different durations of CHX (20 μM) administration, with or without USP7 knockdown. Data are shown as mean ± SEM (**P < 0.01). J, K SAMHD1 expression after different durations of CHX (20 μM) administration, with or without P5091 (20 μM) pretreatment. Data are shown as mean ± SEM (***P < 0.001).

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