Fig. 5: The USP7-SAMHD1 axis modulates cell survival and apoptosis under genotoxic insults. | Oncogene

Fig. 5: The USP7-SAMHD1 axis modulates cell survival and apoptosis under genotoxic insults.

From: De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity

Fig. 5: The USP7-SAMHD1 axis modulates cell survival and apoptosis under genotoxic insults.

A-D HCT116 cells with or without P5091 (20 μM) pretreatment were stimulated with or without cisplatin (5 μM) for 4 h, and then stained with the anti-SAMHD1 or anti-CtIP and anti-γH2AX antibodies, DAPI, respectively, for immunofluorescence analysis. Scale bar, 10 μm. E HCT116 cells with or without P5091 (20 μM) administration were treated with cisplatin (10 μM) for 24 h followed by staining with PI and FITC-Annexin V, and analyzed by FACS; ***P < 0.001. F HCT116 shNC and shUSP7 cells or shUSP7-Flag and shUSP7-Flag-SAMHD1 cells were treated with cisplatin (20 μM) for 24 h followed by staining with PI and FITC-Annexin V, and analyzed by FACS; ***P < 0.001. G HCT116 shNC and shUSP7 cells or shUSP7-Flag and shUSP7-Flag-SAMHD1 cells were treated with cisplatin (5 μM) for 12 h followed by western blot analysis of USP7, Flag, SAMHD1, PARP, Cleaved-PARP, Caspase 3, Cleaved-Caspase 3, γH2AX. H HCT116 shNC and shUSP7 cells or shUSP7-Flag and shUSP7-Flag-SAMHD1 cells were treated with cisplatin at different concentrations for 24 h. Cell viability was assessed by CCK8 assay.

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