Fig. 5: p53-VprBP interaction and p53 nuclear export regulated by S367p and acetylation.
From: VprBP/DCAF1 regulates p53 function and stability through site-specific phosphorylation
A U2OS cells were treated with DMSO and etoposide (40 µM) for 24 h and immunostained with p53S367p, p53 and p53ac antibodies. B H1299 cells were transfected with VprBP and/or p53 expression plasmids for 48 h as indicated on the left and immunostained with anti-p53 antibody. C H1299 cells were transfected with FLAG-VprBP and/or p53 or p536KQ for 48 h. Extracts were prepared in lysis buffer and were subjected to immunoprecipitation using p53 DO-1 antibody. The binding of VprBP to p53 was analyzed by Western blotting. D GST alone or the indicated GST‐p53 fusions were immobilized on glutathione‐Sepharose beads and incubated with His‐VprBP. After washing, bound VprBP proteins were fractionated by SDS/PAGE and examined by Western blotting with anti‐His antibody.
