Fig. 4: IDH1 demonstrates sialidase activity. | Oncogene

Fig. 4: IDH1 demonstrates sialidase activity.

From: Glioma immunotherapy enhancement and CD8-specific sialic acid cleavage by isocitrate dehydrogenase (IDH)-1

Fig. 4

A Crystal structure of isocitrate and NADP+ bound in the active site of IDH1. Dashed yellow lines show hydrogen bonds. B Predicted model of sialic acid in the active site of IDH1. C Predicted model of sialic acid in the active site of IDH1R132H. D Sialic acid release assay of IDH1 relative to V. cholera neuraminidase (VCN), demonstrates in vitro IDH1-mediated sialidase activity against purified CD8β monomer that is largely dependent on NADP+. E IDH1 sialidase activity against CD8β is abrogated by the IDH1R132H mutation.

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