Fig. 5: The SA mutation affects the cell cycle response to irradiation.
From: A DNA-PK phosphorylation site on MET regulates its signaling interface with the DNA damage response
A Upper panels: Western blots of whole cell lysates from the MT, SAMT, YH and SAYH cell lines at the indicated times (hours) after 10 Gy irradiation using antibodies for β-Actin (loading control) and the phosphorylated forms of histone 3 (H3), histone H2AX (γH2AX), Chk1 and Chk2. Lower panels: Quantification of the pH3 signal from the Western blots, normalized to the loading control and to the control conditions. The SA mutation leads to a slightly stronger early response to irradiation but a stronger re-entry into proliferation 24 h after treatment. B Flow cytometry analysis (PI staining) of the cell cycle response to IR at the indicated timepoints after 10 Gy irradiation. The SA mutation prevents proper G2 arrest after treatment (see Fig. S8 for the measurements of all phases). Statistical tests: student’s t-test. Error bars represent the SEM.
