Fig. 6: The SA mutation leads to an increase of abnormal mitoses after IR, resulting in slower proliferation. | Oncogene

Fig. 6: The SA mutation leads to an increase of abnormal mitoses after IR, resulting in slower proliferation.

From: A DNA-PK phosphorylation site on MET regulates its signaling interface with the DNA damage response

Fig. 6: The SA mutation leads to an increase of abnormal mitoses after IR, resulting in slower proliferation.The alternative text for this image may have been generated using AI.

A Representative pictures at 60x magnification of mitoses counted as normal (MT, YH) and abnormal (SAMT, SAYH) (overlay of nuclear staining (DAPI, blue), Alpha-Tubulin (red), Gamma-Tubulin (green); single channel pictures are provided in Fig. S9). B Quantification of abnormal mitoses 24 h after IR (4 Gy) in the MT, SAMT, YH and SAYH cell lines. The SA mutation causes an increase in multipolar mitoses. C CFSE dye dilution assay for the MT, SAMT, YH and SAYH cell lines. The Relative proliferation speed was measured over 4 days after treatment with the indicated doses. The SA mutation leads to a slower proliferation after irradiation. Statistical tests: 2-way anova. Error bars represent the standard deviation (C) or SEM (B).

Back to article page