Fig. 4: The E7 oncoprotein is responsible for the increase in SUR1 expression. | Oncogene

Fig. 4: The E7 oncoprotein is responsible for the increase in SUR1 expression.

From: Exploitation of ATP-sensitive potassium ion (KATP) channels by HPV promotes cervical cancer cell proliferation by contributing to MAPK/AP-1 signalling

Fig. 4: The E7 oncoprotein is responsible for the increase in SUR1 expression.

A, B Relative ABCC8 mRNA expression measured by RT-qPCR in (A) HeLa and SiHa cells and (B) HPV18+ primary keratinocytes co-transfected with E6- and E7-specific siRNA. Samples were normalised against U6 mRNA levels. Successful knockdown was confirmed by analysing E6 and E7 mRNA levels. C, D Expression levels of ABCC8 mRNA measured by RT-qPCR in (C) C33A cells and (D) NHKs transfected with GFP-tagged HPV18 oncoproteins. Samples were normalised against U6 mRNA levels and data is presented relative to the GFP-transfected control. Successful transfection was confirmed by immunofluorescence analysis (not shown). E Relative expression of ABCC8 mRNA in C33A cells stably-expressing HA-tagged HPV18 oncoproteins measured by RT-qPCR. Samples were normalised against U6 mRNA levels. Expression of oncoproteins was confirmed by western blot. F Mean DiBAC4(3) fluorescence levels in C33A cells after transfection of FLAG-tagged HPV18 E7 and treatment with either DMSO or glibenclamide (10 μM). Samples were normalised to the pcDNA3-transfected control. G Mean DiBAC4(3) fluorescence levels in C33A cells after co-transfection of FLAG-tagged HPV18 E7 and SUR1-specific siRNA. Samples were normalised to the pcDNA3/scramble-transfected control. H Mean DiBAC4(3) fluorescence levels in HeLa and SiHa cells after transfection of HPV E7-specific siRNA. Samples were normalised to the scramble control. Bars represent means ± SD of three biological replicates with individual data points displayed. Ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t test).

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