Fig. 3: Impaired tumor growth in Jmjd6+/− mice is macrophage-dependent. | Oncogene

Fig. 3: Impaired tumor growth in Jmjd6+/− mice is macrophage-dependent.

From: JMJD6 in tumor-associated macrophage regulates macrophage polarization and cancer progression via STAT3/IL-10 axis

Fig. 3

Jmjd6+/− and WT mice were given subcutaneous infection of LLC lung cancer cells and tail vein injection of B16F10 melanoma cells. n = 7. a The tumor growth curve of subcutaneous LLC tumors from the two groups. b The weights of subcutaneous LLC tumors from the two groups. c The percentage of CD206+ M2-like TAMs in total TAMs infiltrating into the subcutaneous LLC tumors was analyzed by flow cytometry. IL-10 expression in total TAMs (d) and M2-like TAMs (e) infiltrating into the subcutaneous LLC tumors was analyzed by flow cytometry. f Mouse lungs at 21st day after B16F10 cell injection. g The weight of lungs. h Representative images H&E staining of lungs with B16F10 tumors. i The percentage of total TAMs, M2-like TAMs and M1-like TAMs in immune cells infiltrating into the lungs with B16F10 tumors was analyzed by flow cytometry. j IL-10 expression in M2 TAMs infiltrating into the lungs with B16F10 was analyzed by flow cytometry. k–n Mice were inoculated with tumor cells via tail vein 24 h after treatment of 200 μL clodronate liposomes (CLO Lipo). CLO Lipo was injected every four days to maintain the depletion of macrophages in vivo. Ctrl Lipo was used as control. n = 4. Flow cytometry results showed that CLO Lipo depleted TAMs (CD45+ CD11b+ F4/80+) (k) including M2-like TAMs (CD45+ CD11b+ F4/80+ CD206+) (l). m The effect of CLO Lipo treatment on the development of LLC subcutaneous tumors. Statistical diagram of tumor volume at the end of the experiment. n The effect of CLO Lipo treatment on B16F10 lung metastases. Statistical diagram of tumor foci numbers per lung at the end of the experiment. Data are shown as mean ± SD. ns: no statistical difference, *P < 0.05, **P < 0.01, ***P < 0.001.

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