Fig. 5: shRNA-induced JMJD6 inhibition in THP-1 cells alters macrophages phenotypes.

a JMJD6 protein level was detected in THP-1 cells after shRNA interference. b Tumor-CM stimulation induced the increase of JMJD6 expression in the control groups, but did not change the low expression of JMJD6 in sh-J6 THP-1 cells. c CD206 on the TC-Mφ surfaces was analyzed by flow cytometry, n = 3. d IL-10 secretion in the culture medium of TC-Mφ was detected by CBA, n = 3. e RNA-seq heatmap of differentially expressed genes among Mock, sh-Scr and sh-J6 TAM-like THP-1 cells. 3139 differentially expressed genes were clustered by sample (column) and gene (row). f Venn diagram represented DEGs from two comparisons of sh-J6 vs Mock and sh-Scr vs Mock. g Heatmap of 87 genes related to M1 or M2 activation grouped by functional characteristics. Data were normalized to sh-Scr. h–j NOD-SCID mice were inoculated subcutaneously with 5 × 10⁶ human lung cancer H460 cells resuspended in conditioned medium (CM) of Mock, sh-Scr or sh-J6 TAM-like THP-1 cells, RPMI-1640 medium without additive agents (Control). The experiment was terminated on the 21st day. n = 6. h Schematic diagram. i Tumor growth curve. j Statistical diagram of tumor volumes at the end of the experiment. Data represent mean ± SD. ns: no statistical difference, *P < 0.05, **P < 0.01, ***P < 0.001.