Fig. 2: HUWE1 silencing causes PARPi resistance in BRCA1 exon 11 mutant cells but not in wild type, BRCA2m or BRCA1 knockout cells.
From: A HUWE1 defect causes PARP inhibitor resistance by modulating the BRCA1-∆11q splice variant
A, B siHUWE1 causes talazoparib and olaparib resistance in BRCA1 exon11 mutant SUM149 cells. Cells were transfected with siRNAs and 48 h later, exposed to PARP inhibitors for five days. Cell survival was measured using CellTiter-Glo. C, D siHUWE1 causes talazoparib and olaparib resistance in BRCA1 exon11 mutant UWB1.289 cells. E HUWE1 silencing does not cause talazoparib resistance in BRCA1 wild type CAL51 cells. F HUWE1 silencing does not cause talazoparib resistance in BRCA2 mutant Capan1 cells. G, H Silencing of HUWE1 does not cause pronounced olaparib resistance in BRCA1 knockout RPE-1 cells (G), nor in parental RPE-1 p53/hTERT cells (H). Error bars represent standard deviation, n ≥ 3.
