Fig. 6: SNAI2 depletion inhibits the enhanced migration, invasion and metastasis capability by FBXO28 knockdown.
From: FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation
A Hep3b cells stably expressing shCtrl, shFBXO28 alone or in combination with shSNAI2 were subjected to transwell assays. Scale bar: 10 μm. Data were presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA). B Hep3b cells (5 × 106) stably expressing shCtrl, shFBXO28 alone or in combination with shSNAI2 were injected into NOD/SCID mice via tail vein, and the number of lung metastatic nodules was counted after mice were sacrificed at 8 weeks after injection (n = 4–5). Scale bar: 4 mm, *P < 0.05 (one-way ANOVA). C Hematoxylin and eosin staining of the indicated lung metastatic nodules. The scale bars in left and right panel represent 500 μm and 100 μm, respectively.
