Fig. 6: Mettl1 inhibition disrupts autophagy and decreases viability in PCa cells in vivo.
From: N7-methylguanosine methylation of tRNAs regulates survival to stress in cancer
Immunostained images (A) and quantification (B) of LC3 levels, γH2AX+ cells, cleaved-Caspase 3+ (Cl.Casp3) cells, in PCa from PtencKO/Met+/+ and PtencKO/Metfl/fl mice. Arrows indicate positive cells. Mean ± SD, n = 4 mice, 10 images per mouse. C Prostate tumour volumes of PtencKO/Met+/+ (M+/+) and PtencKO/Metfl/fl (Mfl/fl) mice. Mean ± SD, n ≥ 5. D Immunostained images of markers of luminal (K8), and basal cells (K14), proliferation (Ki67), and Hematoxylin and Eosin staining of tumours from PtencKO/Met+/+ and PtencKO/Metfl/fl mice. E Quantification of Ki67+ cells in mouse PCa. Mean ± SD, n = 4 mice, 10 images per mouse. Cell population distributions analysed by flow cytometry (F) and quantification (G) of prostatic cell subpopulations from healthy (WT), PtencKO/Met+/+ and PtencKO/Metfl/fl mice according to cell surface markers Sca1 and CD49f. Mean ± SD, n = 5. Scale bar: 25 μm (A), 50 μm (D). Stats: Mann–Whitney test (B, C, E), Two-way ANOVA (G) *p < 0.05, **p < 0.01, ****p < 0.0001.
