Fig. 3: Knockdown of VPS35 induces G1/S phase arrest in GC cells. | Oncogene

Fig. 3: Knockdown of VPS35 induces G1/S phase arrest in GC cells.

From: VPS35 promotes gastric cancer progression through integrin/FAK/SRC signalling-mediated IL-6/STAT3 pathway activation in a YAP-dependent manner

Fig. 3

A The cell cycle distribution of VPS35-overexpressing HGC-27 and NCI-N87 cells was analysed by flow cytometry. B The cell cycle distribution of MGC-803 and BGC-823 cells subjected to VPS35 knockdown was analysed by flow cytometry. C–F VPS35-overexpressing cells were collected at 0 and 24 h after release from synchronisation with 2 mmol/L thymidine (C, D) or 0.8 μM nocodazole (E, F). G–J VPS35 knockdown cells were collected at 0 and 24 h after release from synchronisation with 2 mmol/L thymidine (G, H) or 0.8 μM nocodazole (I, J). The cell cycle distributions were examined by flow cytometry analysis. K, L The expression of CDK4/6, cyclin D1, p-Rb, Rb, and VPS35 was detected by Western blotting in VPS35-overexpressing HGC-27 and NCI-N87 cells (K) and in MGC-803 and BGC-823 cells subjected to VPS35 knockdown (L). *p < 0.05, **p < 0.01.

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