Fig. 1: The N-terminal dileucine motif is essential for sorting p53 into the exosomes.

A Western blot analysis of the exosomes isolated from p53^null-expressing H1299 (Negative control) and p53^R273H-endogenous expressing HT-29 cells, with Alix, tsg101 and CD63 considered positive markers and calnexin considered a negative marker of the exosomes. nanoparticle tracking analysis (B) and transmission electron microscopy analysis (C) of the exosomes isolated from HT-29 (p53^R273H) cells (C; scale bar: 100 nm). D Schematic representation of the mutations in p53. E Expression of Flag-p53^WT, p53^DelN94, and p53^DelC101 in the exosomes isolated from HEK293 cell culture supernatant. CD63, Alix, and tsg101 were considered positive markers, while calnexin was considered negative marker for exosomes. F Expression of Flag-p53^WT and p53^LL25AA in the exosomes isolated from HEK293 cell culture supernatant. CD63, Alix, and tsg101 were considered positive markers, while calnexin was considered negative marker for exosomes.