Fig. 2: UBE2J1-loss promotes antiandrogen resistance in vivo. | Oncogene

Fig. 2: UBE2J1-loss promotes antiandrogen resistance in vivo.

From: UBE2J1 is the E2 ubiquitin-conjugating enzyme regulating androgen receptor degradation and antiandrogen resistance

Fig. 2

A Tumor growth curve of xenografted LNCaP/AR cells transduced with Cas9 and annotated guide RNAs in castrated mice. ENZ denotes enzalutamide treatment at 10 mg/kg from day 3 of grafting. The number (n) of tumors in each group was annotated. p values were calculated using two-way ANOVA with Bonferonni multiple-comparison test. Schematic figure was created with BioRender.com. B, C Immunohistochemical staining of B UBE2J1 and C Ki67 proteins on representative sgNT and sgUBE2J1 xenograft tumor slides from castrated mice in (A), scale bar represents 50 µm. Statistical analysis of representative pictures (n = 4–6) is presented, and p values were calculated using two-tailed t-test. D Tumor growth curve of xenografted LNCaP/AR cells transduced with Cas9 and annotated guide RNAs in intact mice. Veh denotes 0.5% CMC + 0.1% Tween 80 + 5% DMSO. The number (n) of tumors in each group was annotated. p values were calculated using two-way ANOVA with Bonferonni multiple-comparison test. Schematic figure was created with BioRender.com. E, F Immunohistochemical staining of E UBE2J1 and F Ki67 proteins on sgNT and sgUBE2J1 xenograft tumor slides from intact mice in (D), scale bar represent 50 µm. Statistical analysis of representative pictures (n = 4–6) is presented, and p values were calculated using two-tailed t-test. For all panels, mean ± s.e.m. is presented.

Back to article page