Fig. 4: UBE2J1-loss leads to increased AR half-life and AR accumulation. | Oncogene

Fig. 4: UBE2J1-loss leads to increased AR half-life and AR accumulation.

From: UBE2J1 is the E2 ubiquitin-conjugating enzyme regulating androgen receptor degradation and antiandrogen resistance

Fig. 4

A ChIP-qPCR of the genomic loci of canonical AR target genes in LNCaP/AR cells transduced with Cas9 and annotated guide RNAs, treated with Veh (−DHT) or DHT (+DHT) in charcoal stripped serum (CSS) media. B ChIP-qPCR of the genomic loci of canonical AR target genes in LNCaP/AR cells transduced with Cas9 and annotated guides RNAs, treated with Veh (DMSO). For (A), (B), n = 3 independently treated cell cultures and mean ± s.e.m. is presented. C Schematic figure represents the experimental setting of cycloheximide-based protein half-life assay. Schematic figure was created with BioRender.com. D Western blots represent AR and Actin proteins in LNCaP/AR cells transduced with Cas9 and annotated guide RNAs treated with cycloheximide (CHX) (30 µg/mL) for the annotated hours. E Dot plot represents quantification of western blot in (D) measuring AR half-life. F Western blot represents AR and Actin protein levels in LNCaP/AR cells transduced with Cas9 and annotated guide RNAs in the presence and absence of a proteasome inhibitor (10 µM Epoxomicin) for 6 h.

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