Fig. 5: MYCN, SNRPD3 and PRMT5 form a protein complex to enhance SNRPD3 methylation. | Oncogene

Fig. 5: MYCN, SNRPD3 and PRMT5 form a protein complex to enhance SNRPD3 methylation.

From: MYCN and SNRPD3 cooperate to maintain a balance of alternative splicing events that drives neuroblastoma progression

Fig. 5: MYCN, SNRPD3 and PRMT5 form a protein complex to enhance SNRPD3 methylation.

A Representative western blot of MYCN, PRMT5, SNRPD3 and SNRPD3 protein arginine methylation (methylated SNRPD3) expression in SHEP.tet21n cells following treatment with either vehicle or Dox at 48 and 72 h. B Densitometry analysis of SNRPD3 protein arginine methylation, PRMT5 and SNRPD3 protein expression in SHEP.tet21n cells following treatment with either vehicle or Dox at 48 and 72 h. C Representative western blot for endogenous PRMT5 and SNRPD3 after immunoprecipitation of SNRPD3 from SK-N-BE(2)-C and KELLY cells. Five percent of the cell lysate was loaded as input. D Representative western blot for endogenous SNRPD3, PRMT5 and MYCN after immunoprecipitation of MYCN from SK-N-BE(2)-C or KELLY cells. Five percent of the cell lysate was loaded as input. All results represent n = 3 independent biological replicates, mean ± SEM, where the P value was determined through a two-way ANOVA, with Tukey’s multiple comparison tests.

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