Fig. 3: GSK3β inhibition enhances peroxisomal biogenesis through PPARα to induce LD accumulation.
From: PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer
A Relative normalized expression of indicated genes relating to PPAR signaling (PPARα, β, γ), peroxisomal biogenesis (PEX3, PEX5, and PEX7), and Lipid droplet growth (TIP47) in PC3LN4 and PC3LN4crisprPIM1 cells. B Western blotting of TIP47 expression and GSK3β S9 phosphorylation in the indicated cell lines. C Representative images of peroxisomal marker catalase (CAT) in PC3TripzPIM1 cells following doxycycline treatment (50 ng/ml, 24 h). D Quantification of CAT puncta size and number per nuclei (n > 30 cells/group). E Representative images of basal CAT staining in the indicated cell lines. F Quantification of CAT puncta size and number per nuclei (n > 30 cells/group). G Representative images of CAT in PC3 cells following CHIR treatment (50 nM, 24 h). H Quantification of CAT puncta size and number per nuclei (n > 30 cells/group). For (C), (E), and (G), CAT is in green, and nuclei in blue (Dapi). Scale bars, 50 µm. n = 3, mean ± SEM. *p ≤ 0.05, ***p ≤ 0.001.
