Fig. 4: PPARα inhibition abrogates the effect of PIM1 LD accumulation and prostate cancer proliferation.
From: PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer
A Representative images of LDs in PC3LN4pCIP (basal) and PC3LN4hPIM1 (basal and with PPARα inhibitor, GW6471 treatment, 4 µM, 24 h) cells. LDs are in red (Oil Red O) and nuclei in blue (Hematoxylin). Scale bars, 50 µm. B Quantification of LD size and number per nuclei (n > 27 cells/group). n = 3, mean ± SEM. ***p ≤ 0.001. C Representative images of crystal violet staining of PC3LN4pCIP and PC3LN4hPIM1 cells treated with DMSO or PPARα inhibitor (GW6471, 4 µM, 72 h). D Quantification of crystal violet staining representing fold change in cell viability. n = 3, mean ± SEM. **p ≤ 0.01, ***p ≤ 0.001.
