Fig. 5: Analysis of lipid composition with PIM1 induction in prostate cancer.
From: PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer
PC3Tripz-PIM1 cells were treated with DMSO or doxycycline (50 ng/ml, 24 h) and lipids were extracted and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A Levels of various lipids in PC3Tripz-PIM1 cells. Raw values were normalized, and fold change vs DMSO was calculated to generate relative normalized ion intensity values. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01. B Relative normalized ion intensity values for most abundant TG species detected. Raw values were normalized, and fold change vs DMSO was calculated to generate relative normalized ion intensity values. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01. C Relative normalized ion intensity values for most abundant PC species detected. Raw values were normalized, and fold change as vs DMSO was calculated to generate relative normalized ion intensity values. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01. D Relative normalized ion intensity values for most abundant LPC species detected. Raw values were normalized, and fold change as vs DMSO was calculated to generate relative normalized ion intensity values. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01.
