Fig. 7: PIM inhibition reduces LD accumulation and tumor growth in vivo.
From: PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer
A Representative images of LDs in PC3LN4 xenograft tumors following indicated treatment. LDs are in red (Oil Red O) and nuclei in blue (Hematoxylin). Scale bars, 50 µm. B Quantification of average LD size and number. n = 3, mean ± SEM. **p ≤ 0.01, ***p ≤ 0.001. C Tumor volume (mm3) determined over time. n = 8, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01. D Representative images of tumor sections (4 micrometers) examined by H&E and immunohistochemical analysis. Scale bars, 50 µm. E Quantification of immunohistochemistry. F Western blot analysis of tumor samples alongside indicated cells exhibiting similar patterns in nitro and in vivo following PIM inhibition. G Heat map showing downregulation of DG and TG levels in serum samples isolated from indicated mice. H working model describing the role of PIM1 kinase in regulating LD accumulation through GSK3β-PPARα axis to promote prostate cancer survival and proliferation.
