Fig. 1: YAP knockout inhibited the proliferation, migration and invasion of human esophageal squamous cells. | Oncogene

Fig. 1: YAP knockout inhibited the proliferation, migration and invasion of human esophageal squamous cells.

From: The Hippo-YAP signaling pathway drives CD24-mediated immune evasion in esophageal squamous cell carcinoma via macrophage phagocytosis

Fig. 1: YAP knockout inhibited the proliferation, migration and invasion of human esophageal squamous cells.The alternative text for this image may have been generated using AI.

A PCR product from genomic DNA of selected clone4(#4) and clone5(#5) showing homozygous deletion of YAP in EC9706. B Sanger sequencing of clone4 and clone5 showing deletion of part of the YAP coding sequence. C PCR product from genomic DNA of selected clone3(#3) and clone12(#12) showing homozygous deletion of YAP in ECA109. D Sanger sequencing of clone3 and clone12 showing deletion of part of the YAP coding sequence. E Immunoblotting analysis showing CRISPR-mediated deletion of YAP in ESCC cell lines. F Cell viability was determined at indicated time points using CCK8 assay. Data are shown as mean ± s.e.m. ***P < 0.001. G Clone formation efficiency of WT and YAP KO cells. H, I YAP knockout inhibited the migration and invasion of different human esophageal squamous cells as determined by Transwell assays. Scale bars, 100 μm. J, K Quantification of migration and invasion cells. ***P < 0.001. L–N Representative tumor size, quantification of tumor weight and tumor growth curves from xenograft mouse models.

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