Fig. 2: TKT depletion suppresses DSB repair in HCC. | Oncogene

Fig. 2: TKT depletion suppresses DSB repair in HCC.

From: TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma

Fig. 2

Schematic of the DR-GFP (A) and EJ5-GFP (C) reporter systems and relative HR (B) and NHEJ (D) repair efficiency in U2OS cells after being transfected the indicated siRNAs. Immunoblots of TKT and quantitative PCR (qPCR) analysis of BRCA1 (B) or 53BP1 (D) in U2OS cells after being transfected with the indicated siRNAs. ACTIN is used as a loading control. siBrca1 and si53bp1 are used as a positive control. Statistical analyses are presented as the mean±s.d. from three independent experiments. **p < 0.01; ***p < 0.001; ****p < 0.0001 of Student’s t test. Schematic of the HR (E) and NHEJ (G) reporter systems and relative HR (F) and NHEJ (H) repair efficiency in HCCLM3 cells with TKT stable knockdown. Immunoblots of TKT (F–H) in HCCLM3-shCtrl or shTKT cells. ACTIN is used as a loading control. Statistical analyses are presented as the mean±s.d. from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 of Student’s t test.

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