Fig. 4: AR expression levels in OS cell lines. | Oncogene

Fig. 4: AR expression levels in OS cell lines.

From: RNA-sequencing predicts a role of androgen receptor and aldehyde dehydrogenase 1A1 in osteosarcoma lung metastases

Fig. 4

A The mRNA expression levels of ALDH1A1 and AR were detected by qRT-PCR for the related OS cell lines SaOS-2 and SaOS-LM2. Prostate cancer cell lines PC3 and C4-2 were used as negative and positive controls, respectively. SaOS-2 was found to express high levels of AR and relatively low levels of ALDH1A1, while SaOS-LM2 was discovered to express low levels of AR and high levels of ALDH1A1. Data are expressed as mean ± SD (n = 3, experiment was performed in triplicate). B Western blotting also revealed AR expression at the protein level in both OS cell lines. GAPDH served as loading control, while PC3 and C4-2 served as negative and positive controls, respectively for AR.

Back to article page