Fig. 2: CAFs reduce estrogen receptor activity in co-cultures in part via secreted factors. | Oncogene

Fig. 2: CAFs reduce estrogen receptor activity in co-cultures in part via secreted factors.

From: Cancer-associated fibroblasts rewire the estrogen receptor response in luminal breast cancer, enabling estrogen independence

Fig. 2

The CAF2 cell line affects ER-α activity in luminal breast cancer cell lines MCF7, T47D and BT474 clone 5 cells in direct co-cultures for 96 h (A–C), in transwell (TW) co-cultures for 72 h (D–F) and in CAF2-conditioned media for 48 h (G–I). MCF7 cells were starved of estrogen for 24 h before stimulation with estrogen for 48 h and ER-α-induced luciferase activity measured. Mo = monoculture; Co = co-culture; CM: CAF2 Conditioned Media, E2 = estrogen. Unpaired ordinary one-away ANOVA, Fisher’s LSD multiple comparisons test: *P < 0.05, ***P < 0.001, ****P < 0.0001, ns not significant. Error bars: SEM.

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