Fig. 5: Silencing AF1q in Lan-5 cells mediates apoptosis in a p53-dependent fashion.
From: AF1q is a universal marker of neuroblastoma that sustains N-Myc expression and drives tumorigenesis
A Time course demonstrating p53 induction and inhibition of cell cycle progression after AF1q silencing in Lan-5 cells. GAPDH is a loading control. B Silencing AF1q in Lan-5 cells results in downregulation of the p53 inhibitor, MDM2 (in duplicate). C Efficient silencing of p53 in Lan-5 cells maintains cell cycle progression (as shown by sustained H3-S10P expression) and prevents apoptosis (as shown by reduced cleavage of PARP and caspase-3) despite silencing of AF1q (in triplicate). All cells were analyzed 96 h after infection with shRNA carrying lentiviruses.
