Fig. 6: Silencing AF1q leads to downregulation of N-Myc in MYCN amplified neuroblastoma.
From: AF1q is a universal marker of neuroblastoma that sustains N-Myc expression and drives tumorigenesis
A Immunoblot demonstrating the effects of AF1q silencing on N-Myc protein abundance in MYCN amplified Kelly and Lan-5 cells and C-Myc protein abundance in MYCN non-amplified SH-SY5Y neuroblastoma cells harvested 96 h after AF1q knockdown. N-Myc downregulation in Kelly and Lan-5 cells coincides with reduced proliferation as shown by H3-S10P levels (in duplicate). B N-Myc downregulation in Kelly cells harvested 120 h after AF1q knockdown (in triplicate). C, D Side by side comparison of the effectiveness of 2 different shRNAs targeting AF1q locus: shRNA-1 targets 3’UTR and shRNA-2 is against AF1Q coding sequence. Lan-5 cells (in triplicate, Part C) were harvested 96 h after infection. D Kelly cells (in duplicate) were harvested 120 h post transduction.
