Fig. 4: Procaspase-8 may undergo symmetric di-methylation.
From: Arginine methylation of caspase-8 controls life/death decisions in extrinsic apoptotic networks
A Three types of arginine methylation are shown schematically. B HeLa-CD95-C8-KO cells transfected with pcDNA3 (vector), procaspase-8a-R233H-pcDNA3 (R233H), procaspase-8a-R435Q-pcDNA3 (R435Q) and procaspase-8a-WT-pcDNA3 (WT) as well as non-transfected (KO) were treated with 1000 ng/mL of CD95L (+) for one hour (h). This was followed by SYM-10-IP with subsequent Western Blot analysis. In these experiments, immunoprecipitation has been carried out without immobilization, which we designate as IP. Lysates (Input), IPs and bead-only control (B) are shown. One representative Western Blot analysis out of three independent experiments is shown. C HeLa-CD95-C8-KO cells transfected with pcDNA3 (vector), and procaspase-8a-WT-pcDNA3 (WT) as well as non-transfected (KO) were treated with 1000 ng/mL of CD95L (+) for one hour in the presence or absence of EPZ inhibitor. This was followed by SYM-10-IP with subsequent Western Blot analysis. Lysates (Input), IPs and bead-only control (B) are shown. One representative Western Blot analysis out of three independent experiments is shown. IP immunoprecipitation, B beads-only pulldown, s.e. short exposure, l.e. long exposure, v vector. B, C Non-specific signals are marked with *. The signal for the heavy chain of antibody used for IP is marked with IgGH.
