Fig. 5: MCPIP1 emerges as a potential downstream regulator of SUV39H1.

A Heatmap showed the common top 20 upregulated genes after SUV39H1 inhibition by chaetocin treatment or genetic knockdown. B qRT-PCR analysis confirmed the upregulation of MCPIP1 in NB cells after chaetocin treatment at 25 nM or 50 nM for 48 h. C qRT-PCR analysis confirmed the upregulation of MCPIP1 in NB cells after SUV39H1 genetic knockdown with shRNAs. D WB analysis confirmed the upregulation of MCPIP1 in NB cells after chaetocin treatment for 48 h or genetic knockdown of SUV39H1 with shRNAs. E Spearman correlation analysis of 143 NB samples based on the TARGET database indicated the negative correlation between MCPIP1 and SUV39H1. F qRT-PCR analysis revealed the expression level of MCPIP1 in 5 NB cell lines as compared to the normal HEK293T cell. G R2 online website platform indicated the association of MCPIP1 with the prognosis of NB patients, and the cut-off was determined using the scanning method. H qRT-PCR confirmed the transfection efficiency of overexpressing MCPIP1 in NB cells after transfection for 48 h. I WB analysis validated the transfection efficiency of overexpressing MCPIP1 transfection for 72 h in NB cells. J CCK-8 analysis identified the proliferation abilities of NB cells after MCPIP1 overexpression at 0, 24, 48, 72 and 96 h. K Colony formation analysis of NB cells after MCPIP1 overexpression (14 days for SK-N-SH and 12 days for SK-N-BE (2)). L Changes of apoptosis in NB cells after overexpressing MCPIP1. M Changes of the cell cycle distribution based on MCPIP1 overexpression. B, C, M The two-way ANOVA was performed for statistical analysis. F, J The one-way ANOVA with multiple comparisons to the control was performed for statistical analysis. H, K, L The t-test was performed for statistical analysis. The experiments were repeated three times with similar results. Data were shown as the mean ± SEM, ns: non significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.