Fig. 5: Effect of KCNN1 knockdown on membrane potential and calcium flux.

A Measures of the plasma membrane depolarization in A-673 cell line stably transduced with shRNA control cells (shRNA CT) or three shRNA cells targeting KCNN1 after 7 days of doxycycline treatment (0.5 µg/mL). (N = 48 for shRNA CT and shRNA 908, N = 46 for shRNA 511, N = 45 for shRNA 576). B Membrane potential measures of shRNA CT or shRNA 908 spheroids treated 7 days with doxycycline (0.5 µg/mL) (N = 7). C Plasma membrane depolarization measures in A-673 cells treated with the SK1 channel activator GW542573X (N = 50). D Effect of KCNN1 knockdown on SOCE in A-673 cell line stably transduced with shRNA control (shRNA CT) or three shRNA targeting KCNN1 and the relative fluorescence measurement of SOCE induced by TG (N = 26). E Effect of KCNN1 knockdown on CCE using Mn²⁺ quenching assay and the normalized Mn²⁺ quenching slope (N = 21 for shRNA CT, N = 20 for shRNA 511 and N = 22 for shRNA 908 and shRNA 576). F Effect of the SK1 channel activator GW542573X on SOCE in A-673 cells and the relative fluorescence measurement of SOCE induced by TG in GW542573X conditions (N = 14 for Control and N = 21 for G542573X condition). G Effect of GW542573X on CCE using Mn²⁺ quenching assay and the normalized Mn²⁺ quenching slope (N = 28 for Control and N = 20 for GW542573X condition). *p < 0.05; **p < 0.01; ***p < 0.001 (boxes indicate the first and third quartiles—and the median inside—of the different measures. The whiskers indicate the fifth and the ninety-fifth percentiles. A, D, E Anova test; B, F, G Mann–Whitney test and C: Unpaired t-test).