Fig. 2: Large polyploid tumor cells are induced following chemotherapy exposure in MDA-MB-231 and PC3 cell lineages.
From: Polyploid cancer cells reveal signatures of chemotherapy resistance
A Experimental schematic for in vitro investigation of surviving polyploid cells. B Representative images in bright field (left) and DAPI (right) channels for PC3 control with a polyploid cell present (left), MDA-MB-231 cisplatin 10 days post treatment (DPT) enriched polyploid (middle), and PC3 docetaxel 10 DPT enriched polyploid (right) conditions. Scale bars are set to 20 µM. C Nuclear diameter for MDA-MB-231 control (DMSO), 10 DPT cisplatin, 1 DPT docetaxel, 10 DPT cisplatin, and 10 DPT docetaxel post treatment recovery. D Nuclear diameter for PC3 control (DMSO), 1 DPT cisplatin, 1 DPT docetaxel, 10 DPT cisplatin, 10 DPT docetaxel post treatment recovery, 24 DPT cisplatin recovery, 10 DPT cisplatin retreated, and 10 DPT docetaxel retreated. Retreated conditions were initially treated with cisplatin and recovered for 10 days. Teal box on docetaxel DPT 10 indicates the condition where single cell progeny (progeny-1 and progeny-2) originated from. E Nuclear diameter of PC3 control parental and progeny-1 cells, and progeny-1 10 DPT recovered cells. All treated conditions in C–E, and progeny-1 DMSO cells, are significantly larger in nuclear diameter compared to DMSO controls (Wilcoxon rank sum; p < 0.01).
