Fig. 4: K26 SUMOylation-defective mutation of YBX1 contributed to YBX1 promoting roles in CRC cell proliferation, migration and tumorigenesis.

A Flag-YBX1^K26R, or Flag-YBX1^4KR overexpression had influence on p-AKT level in YBX1-knockdown CRC cells. B Cell viability was compared in Flag-YBX1^K26R or Flag-YBX1^4KR-expressing CRC cells under the endogenous YBX1 knockdown. All data presented as the means ± SD of three independent experiments. C Cell proliferation capacity was compared in Flag-YBX1^K26R or Flag-YBX1^4KR-expressing CRC cells under the endogenous YBX1 knockdown. Representative images of the colony formation of the indicated cells in top and quantification of clone numbers in bottom. All data are presented as the means ± SD of three independent experiments. D Cell migration capacity was compared in Flag-YBX1^K26R or Flag-YBX1^4KR-expressing CRC cells under the endogenous YBX1 knockdown. Representative images of the wound healing of the indicated cells in top and quantification of clone numbers in bottom. All data are presented as the means ± SD of three independent experiments. Scale bar: 100 μm. E–G The subcutaneous tumor capacity was compared in Flag-YBX1^K26R or Flag-YBX1^4KR-expressing CRC cells under the endogenous YBX1 knockdown. Tumor images (E), volume (F), and weight (G) were shown. Data were presented as the mean ± SD (n = 5 mice). Control Flag-vector, sh-YBX1 short hairpin RNA targeting to YBX1. *p < 0.05, **p < 0.01, ***p < 0.001.