Fig. 6: SENP1 and YBX1 were upregulated in CRC specimens, while YBX1 SUMOylation level was negatively correlated with the SENP1 protein level and indicated good prognosis of CRC patients. | Oncogene

Fig. 6: SENP1 and YBX1 were upregulated in CRC specimens, while YBX1 SUMOylation level was negatively correlated with the SENP1 protein level and indicated good prognosis of CRC patients.

From: SENP1-mediated deSUMOylation of YBX1 promotes colorectal cancer development through the SENP1-YBX1-AKT signaling axis

Fig. 6

A Representative images (left) and quantitative analysis (right) of SENP1 expression profiling in CRC tissues and paired normal tissues (n = 25) by IHC staining. Scale bars: 50 μm. Statistical analysis was performed using the paired two-tailed Student’s t test. B Representative images (left) and quantitative analysis (right) of YBX1 expression profiling in CRC tissues and paired normal tissues (n = 25) by IHC staining. Scale bars: 50 μm. Statistical analysis was performed using the paired two-tailed Student’s t test. C Expression variance of SENP1 and YBX1 in CRC tissues and normal tissues of CRC patients using UALCAN portal analysis. D IP and immunoblotting analysis with indicated antibodies in 6 CRC tissues (T) and paired normal tissues (N). Input, whole cell lysates. SUMOyaltion of immunoprecipitated YBX1 was determined and normalized to YBX1 protein (n = 6 samples). E–G Overall survival based on YBX1 expression among all patients or in stratified groups according to SENP1 expression using Kaplan–Meier Plotter portal analysis. ***p < 0.001, ****p < 0.0001. S-YBX1 SUMOylated YBX1, IP immunoprecipitation, IB immunoblot, Input Same account of cell lysate to load.

Back to article page