Fig. 1: SUMOylation inhibition potentiates Dex-induced cell cycle arrest. | Oncogene

Fig. 1: SUMOylation inhibition potentiates Dex-induced cell cycle arrest.

From: SUMOylation inhibition potentiates the glucocorticoid receptor to program growth arrest of acute lymphoblastic leukemia cells

Fig. 1

A Cell proliferation as assessed by MTS assays. Bars represent mean of 5 replicates with SD. Asterisks denote statistical significance calculated with Two-way ANOVA with Bonferroni post hoc test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001. B Immunoblotting of SUMO2/3 or (C) GR and loading control (GAPDH) in NALM6 cells exposed to vehicle control (DMSO-EtOH), 10 nM Dex, 125 nM SUMOi or combination as depicted by + and – signs. Average SUMO2/3 or GR signal from two replicates normalized to GAPDH and further to time point vehicle control is indicated below the samples. D Bar graph of flow cytometric cell cycle analysis. Bars represent mean of 3 replicates with SD. Asterisks denote statistical significance calculated with One-way ANOVA with Bonferroni post hoc test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001. E Representative flow cytometry gating strategy for the cell cycle analysis. F, G Bar graphs of flow cytometric apoptosis analysis for cells in early-stage and late-stage apoptosis stages, respectively. Bars represent mean of 3 replicates with SD. Asterisks denote statistical significance calculated with One-way ANOVA with Bonferroni post hoc test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001.

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