Fig. 5: Transcription factor binding site methylation analysis.

A BRAF V600E mutation was associated with high methylation levels e.g. at GLIS1 binding sites when MSS BRAF V600E tumors were compared to MSS WT tumors. MSS WT B and MSS BRAF V600E C tumors were compared separately to normal samples. Results with P-adj. < 0.05 are marked with green and nonsignificant results are colored with black. For readability, only a subset of the most significant results are labeled in panels A–C, please see Supplementary Table 7 for a comprehensive results list. MAFG is highlighted in red for visualization purposes. D Top 20 TFs with the most significant p-values from the three different models presented in A–C (red outline = P-adj. <0.05; * = several replicates of ChIP-sequencing data).