Fig. 5: Loss of ZNF768 increases radiosensitivity and alters the transcriptional response to irradiation.
From: ZNF768 loss amplifies p53 action and reduces lung tumorigenesis in mice
Weight variations in wild-type and ZNF768 knockout (A) male and (B) female mice after total body irradiation. Mice were subjected to 2 rounds of 4 Gy total body irradiation (arrow) and weight was measured (n = 9-20 animals/group). Data represent the mean ± SEM. Significance was determined by 2-way ANOVA. Tissue weight of wild-type and ZNF768 knockout (C) male and (D) female mice. Mice described in (A, B) were sacrificed 8 weeks after the first round of total body irradiation, and tissue weight was measured (n = 9-20 animals/group). Data represent the mean ± SEM. Significance was determined by 2-tailed, unpaired t-test. *P < 0.05, ****P < 0.0001 versus controls. E qPCR analysis of p53 target genes in the heart of male wild-type and ZNF768 knockout mice 2 and 8 hours after 4 Gy total body irradiation (n = 7 animals/group). Data represent the mean ± SEM. Significance was determined by 2-way ANOVA. **P < 0.01 ***P < 0.001 ****P < 0.0001 versus controls. F Heat-map visualization of differentially expressed genes in the heart of male wild-type and ZNF768 knockout mice 8 h after 4 Gy total body irradiation (Padj < 0.01 and fold change <0.7 and >1.25; n = 6 animals/group). Significance was determined by Wald test with the Benjamini-Hochberg adjustment method.
