Fig. 6: OGP diminishes MDSC differentiation and does not affect CT26 cell proliferation or inflammatory response in vitro. | Oncogene

Fig. 6: OGP diminishes MDSC differentiation and does not affect CT26 cell proliferation or inflammatory response in vitro.

From: Immunomodulatory function of cannabinoid receptor 2 and its agonist osteogenic growth peptide in health and cancer: a study in mice and humans

Fig. 6

Primary murine bone marrow cells were cultured in CT26-conditioned media and L929-conditioned media to generate MDSCs. a Representative flow cytometry plots of PMN-MDSCs (lower right quadrant) frequency in vehicle control (VEH, left) and 10-12 M OGP (right) treated cells grown in media cocktail for 72 h b Relative frequency of PMN-MDSCs (Ly6GhiLy6Cint) expressed as percent of CD45+CD11b+ cells (% parent). Data is representative of three separate differentiation experiments with similar results. c Nitric oxide levels in the supernatant of cells grown in media cocktail for 72 h, treated with 10−12 M OGP, and stimulated with 100 ng/mL LPS for 48 h. d MTT assay of CT26 cells treated with OGP at the indicated concentrations (M) expressed as relative proliferation compared to VEH. e mRNA expression of IL-6 in CT26 cells after OGP treatment for 4 h. n ≥ 3 per group; Student’s t-test or One-way ANOVA, *p < 0.05, ****p < 0.0001.

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