Fig. 4: β-Catenin interacts with MSI2 in myeloid cells. | Oncogene

Fig. 4: β-Catenin interacts with MSI2 in myeloid cells.

From: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells

Fig. 4

A Scatter plots showing MSI2 detection in β-catenin interactomes performed in cytosolic fractions of K562 and HEL cells. Vertical dashed red line indicates the threshold for 2-fold change in protein binding at log2 (=1) relative to IgG Co-IP. The horizontal red line represents threshold for p < 0.05 on log10 scale (=1.3). Highlighted red dots indicate enriched interactions (p < 0.05), green labels highlight position of MSI2, and blue labels highlight position of β-catenin bait. Representative immunoblots showing the level of β-catenin protein present in MSI2 Co-IPs derived from B K562 - RNase A, C K562 + 20 µg/mL RNaseA, D HEL - RNase A and E HEL + 20 µg/mL RNaseA whole cell lysates, ±5µM CHIR99021 overnight. ID immunodepleted lysate.

Back to article page