Fig. 7: K14 regulates TRIM29-induced focal adhesions and bladder cancer migration. | Oncogene

Fig. 7: K14 regulates TRIM29-induced focal adhesions and bladder cancer migration.

From: TRIM29 promotes bladder cancer invasion by regulating the intermediate filament network and focal adhesion

Fig. 7

A, B Re-expression of TRIM29 in the UM-UC14 TRIM29 KO cell line allows recovery of ZYX+ and PXN+ focal adhesion sites, but this effect is abrogated by K14 KD. Vector: expression vector control. T29: TRIM29 re-expression vector. NT: nontargeting siRNA control. K14KD: siRNA targeting K14. Magenta: ZYX or PXN. Yellow: F-Actin. Green: Reintroduced TRIM29. Blue: Hoechst 33342+ Nuclei. Overexpression of TRIM29 increased migration (C) and invasion (D) which was abrogated by knockdown of KRT14 or ZYX. Top row: UM-UC10; bottom row: UM-UC14. Vector: empty expression vector control. OE: overexpression. KD: siRNA mediated knockdown. Data represent the mean + STD. *p < 0.01. ns: not significant for one-way ANOVA.

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